目的 研究黄花草木犀化学成分及其抗氧化活性。方法 DPPH法及β-胡萝卜素漂白等方法筛选黄花草木犀体积分数70%乙醇提取物的抗氧化活性部位,通过硅胶、Sephadex LH-20、制备柱进行分离纯化,根据理化性质和波谱数据鉴定所得化合物的结构,再评价其抗氧化活性。结果 黄花草木犀的乙酸乙酯部位抗氧化活性最显著,从中分离出13个化合物,分别鉴定为山柰酚(1)、木犀草素(2)、橙皮苷(3)、槲皮素(4)、芦丁(5)、芹菜素(6)、水杨酸(7)、香豆素(8)、滨蒿内酯(9)、7-甲氧基香豆素(10)、富马酸(11)、咖啡酸(12)和对羟基苯甲酸-4-O-α-D-吡喃甘露糖-(1→3)-α-L-鼠李糖苷(13);活性研究表明,化合物2、4、7、12对DPPH自由基的清除能力较强,IC50值分别25.89、14.09、28.03和23.04 μg·mL-1;化合物4、7、12、13对ABTS自由基的清除能力较强,IC50值分别为10.48、23.77、22.79和25.20 μg·mL-1。结论 化合物3、7、9、10、11、12、13为首次从该植物中分离得到,经检索化合物13为未经文献报道的新化合物;化合物2、4、7、12对DPPH自由基的清除能力较强;化合物4、7、12、13对ABTS自由基的清除能力较强。
Abstract
OBJECTIVE To study the chemical constituents from Melilotus officinalis(L.) and their anti-oxidant activities. METHODS DPPH Assay and β-carotene bleaching assay were used for screening the fraction of anti-oxidant activity in the 70% ethanol extract of Melilotus officinalis(L.). The isolation and purification were performed by silica,Sephadex LH-20 and preparative columns. The structures of the obtained compounds were identified by physicochemical properties and spectral data. Then their anti-oxidant activities were evaluated. RESULTS =The ethyl acetate fraction from Melilotus officinalis(L.) showed the most significant anti-oxidant activity. Thirteen compounds were isolated and identified as kaempferol(1), luteolin(2), quercetin(3), hesperidin(4), rutin(5), apigenin(6), salicylic acid(7), coumarin(8), scoparone(9), 7-methoxycoumarin(10), fumaric acid(11), caffeic acid(12), and p-hydroxybenzoate-4-O-α-D-mannopyranosyl-(1→3)-α-L-rhamnoside(13).Compounds 2, 4, 7 and 12 exhibited strong scavenging activities against DPPH free radical with the IC50 values of 25.89, 14.09, 28.03, and 23.04 μg·mL-1, respectively; compounds 4, 7, 12 and 13 exhibited strong scavenging activities against ABTS free radical with the IC50 values of 10.48, 23.77, 22.79, and 25.20 μg·mL-1, respectively. CONCLUSION Compounds 3, 7, 9, 10, 11, 12, and 13 are isolated from this plant for the first time. Compound 13 is a new compound not reported in the literature; compounds 2, 4, 7, and 12 have strong ability to scavenge DPPH free radicals; compounds 4, 7, 12, and 13 have strong ability to scavenge ABTS free radicals.
关键词
黄花草木犀 /
橙皮苷 /
水杨酸 /
滨蒿内酯 /
分离鉴定 /
抗氧化活性
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Key words
Melilotus officinalis(L.) /
hesperidin /
salicylic acid /
scoparone /
Identification /
antioxidant activity
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脚注
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基金
吉林省中药保健食品科技创新中心资助(20180623041TC):基于经方、验方的中药保健食品关键技术与产品开发资助(17YJ007)
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